John Z. Metcalfe

Unpacking bedaquiline hetero-resistance: the importance of intermediate profiles for phenotypic drug-susceptibility testing

This paper shows that considering intermediate results in phenotypic drug susceptibility testing can detect bedaquiline heteroresistance at low frequencies, improving resistance determination.

Beating the gold standard: A review of Mycobacterium tuberculosis lysis using bead beating and the need for standardization.

The paper reviews bead beating methods for Mycobacterium tuberculosis lysis, identifies protocol variability, and highlights the need for standardization to improve reproducibility.

URAdime - a tool for analyzing primer sequences in sequencing data to identify dimers and super-amplicons.

URAdime identifies problematic primers in sequencing data by detecting dimers and super-amplicons post hoc.

spotPCR: A Rapid and Efficient Approach for Indexing Individual Template Molecules using Unique Molecular Identifiers

spotPCR improves mutation detection by streamlining UMI tagging during PCR, enhancing sensitivity and specificity without requiring multiple labor-intensive steps.

Circularization of rv0678 for genotypic bedaquiline resistance testing of Mycobacterium tuberculosis

The paper presents streamlined methods for generating circular DNA from the rv0678 gene to improve genotypic bedaquiline resistance testing in Mycobacterium tuberculosis.