Author

Manuel A.F.V. Gonçalves

Leiden University Medical CenterORCID 0000-0002-8111-1073Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Developmental Biology· 1 Neuroscience· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 2 Organism:human· 2 Research Focus:cell type annotation· 1 Research Focus:clustering· 1 Research Focus:differential expression· 1

Frequent Co-Authors

Aat A. MulderiD· 1 shared Arend W. OvereemiD· 1 shared Celine M. RoelseiD· 1 shared Christian Freund· 1 shared Elon H C van Dijk· 1 shared Hailiang MeiiD· 1 shared Ioannis MoustakasiD· 1 shared Jan WijnholdsiD· 1 shared

Activity by Year

2022-2026 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 20 mutual co-author links

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1 shared paperNeuroscience

Aat A. MulderORCID

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2 papers

Recent·Most cited

USH2A High-Capacity Adenoviral Vector Restores usherin, whirlin, and ADGRV1 in Photoreceptor Cells of Retinal Organoids

Rossella Valenzano, Xuefei Lu, Aat A. Mulder, Elon H C van Dijk, Roman I. Koning, Manuel A.F.V. Gonçalves, Jan Wijnholds

This study demonstrates that high-capacity adenoviral vectors can restore USH2A protein expression and its interacting partners in USH2A-defective retinal organoids.

bioRxiv · Sep 18, 2026 · PreprintNeuroscience

Efficient and scalable generation of primordial germ cells in 2D culture using basement membrane extract overlay

Arend W. Overeem, Yolanda W. Chang, Ioannis Moustakas, Celine M. Roelse, Sanne Hillenius, Talia van der Helm, Valérie F. Van Der Schrier, Manuel A.F.V. Gonçalves, Hailiang Mei, Christian Freund, Susana M. Chuva de Sousa Lopes

This paper introduces a method to efficiently generate human primordial germ cell-like cells (hPGCLCs) in 2D culture using basement membrane extract overlay and low BMP4 concentration.

Cell Reports Methods · Jan 1, 2022Peer-reviewedDevelopmental Biology