Pierre Millard

Organizing principles of the cellular electron economy

The paper introduces the concept of the cellular electron economy to quantify and organize electron flow across metabolism in microorganisms, revealing how carbon sources and electron disposal capacity shape metabolic organization.

Small but Mighty: The Surprising Metabolic Range of a Bacterial Microcompartment

This paper demonstrates that heterologous Pdu BMCs in E. coli can metabolize a broader range of diols and triols than previously known, including glycerol and up to 1,2-pentanediol, and shows their potential for biotechnological applications.

Acetate promotes nutritional adaptation in Escherichia coli

This paper shows acetate helps Escherichia coli adapt to nutrient changes by reducing lag time and replenishing metabolic pools.

MultiNMRFit: A software to fit 1D and pseudo-2D NMR spectra

MultiNMRFit is a software tool for analyzing NMR spectra to extract signal parameters, offering flexibility and robustness in metabolomics and isotope labeling studies.

Estimating flux dynamics from metabolite concentration time courses with dynafluxr

The paper introduces dynafluxr, a method to estimate metabolic flux dynamics from metabolite concentration time courses using constrained B-splines, showing it works well on both simulated and experimental data.

Acetate as a metabolic booster for glucose-based bioproduction in Escherichia coli

This study shows acetate can boost bioproduction in glucose-grown E. coli by reducing carbon loss, increasing acetyl-CoA levels, and improving biomass accumulation, validated for mevalonate and 3-hydroxypropionate.

Combining systems and synthetic biology for in vivo enzymology

The paper develops a method to measure enzymatic parameters in vivo using synthetic biology tools and applies it to study carotenoid pathway enzymes in yeast.

PhysioFit: a software to quantify cell growth parameters and extracellular fluxes

PhysioFit quantifies cell growth parameters and extracellular fluxes using flexible growth models and provides a user-friendly software tool for non-computational biologists.

From toxic waste to beneficial nutrient: acetate boosts Escherichia coli growth at low glycolytic flux

This study shows acetate enhances Escherichia coli growth by compensating for reduced glycolytic flux through co-utilization with glucose.

IsoSolve: an integrative framework to improve isotopic coverage and consolidate isotopic measurements by MS and/or NMR

IsoSolve integrates isotopic measurements from MS and NMR to improve isotopic coverage and consolidate data for metabolic network analysis.

Control and regulation of acetate overflow in Escherichia coli

This study develops a kinetic model of E. coli metabolism to explain acetate overflow, showing acetate regulates glucose metabolism and is not a wasteful by-product.

ScalaFlux: a scalable approach to quantify fluxes in metabolic subnetworks

ScalaFlux quantifies metabolic fluxes in subnetworks without requiring external network information, validated on yeast mutants and simulated data.

Interact: Automated analysis of protein-ligand interactions by 1D and 2D NMR

Interact automates analysis of 1D and 2D NMR titration experiments to quantify protein-ligand interactions through peak picking, annotation, and fitting procedures.