Felicity R. Allen

The paper investigates how Cas9-induced large deletions and small indels are regulated, showing their connection through repair mechanisms and locus-specific factors.

This paper identifies position-specific sequence determinants of base editing efficiency and develops a machine learning model to predict editing outcomes for adenine and cytosine editors.

The paper designs a smaller genome-wide CRISPR-Cas9 library with reduced size while maintaining sensitivity and specificity, enhancing its applicability in complex models.

This paper introduces a minimized double guide RNA library for CRISPR/Cas9 screens, reducing scale by fivefold while maintaining performance.

JACKS improves hit identification in CRISPR/Cas9 screens by jointly analyzing data from the same guide RNA library, reducing required cell numbers by 2.5-fold.

The paper predicts Cas9-induced mutation outcomes based on surrounding DNA sequence using data from over 1 billion mutational outcomes.

The paper identifies an ISRIB-binding site in eIF2B using cryo-EM and CRISPR/Cas9, showing its role in regulating translation through an allosteric mechanism.