Francesca Maria Bosisio

Mapping tumor microenvironment heterogeneity of human melanoma metastases across distant organs

This study maps the heterogeneity of human melanoma metastases across brain, liver, and lung using single-nucleus RNA sequencing, revealing organ-specific immune and stromal adaptations.

The normal human lymph node cell classification and landscape defined by high-dimensional spatial proteomics.

This study classifies and maps cell types in normal human lymph nodes using high-dimensional spatial proteomics, revealing novel cell subsets and interactions.

Longitudinal tumor ecosystem mapping defines glioblastoma treatment trajectories

This study maps glioblastoma tumor ecosystems over time using spatial single-cell profiling to identify prognostic subgroups and treatment responses.

CLEAR-AF: Improved Autofluorescence Subtraction for Multiplexed Tissue Imaging via Polar Transformation and Gaussian Mixture Models

CLEAR-AF improves autofluorescence subtraction in multiplexed tissue imaging by using polar transformation and Gaussian Mixture Models, enhancing specificity, sensitivity, and reproducibility.

CONCLAVE: CONsensus CLustering with Annotation-Validation Extrapolation for cyclic multiplexed immunofluorescence data

CONCLAVE improves cell annotation in cyclic multiplexed immunofluorescence data by using consensus clustering and validation, enhancing accuracy and reproducibility.

COLLAGE: COnsensus aLignment of muLtiplexing imAGEs

COLLAGE improves image alignment for multiplexed immunohistochemistry by breaking alignment into smaller puzzles, enabling parallel processing and reducing errors.

QUAL-IF-AI: Quality Control of Immunofluorescence Images using Artificial Intelligence

QUAL-IF-AI is a deep learning tool that detects and manages artifacts in multiplex immunofluorescence images, achieving high accuracy and IoU across common artifact types.

Autologous micrograft accelerates endogenous wound healing response via ERK-induced cell migration

This study shows that autologous micrograft therapy accelerates wound healing by enhancing fibroblast migration through ERK pathway activation and increased matrix metalloproteinase activity.

The landscape of S100B+ and HLA-DR+ dendritic cell subsets in tonsils at the single cell level via high-parameter mapping

The paper identifies and characterizes novel dendritic cell subsets in tonsils using high-parameter imaging and bioinformatics, revealing spatial interactions with T and B cells.