The paper expands and optimizes the AID2 system for use in Candida species, including clinical isolates and C. auris, enhancing its utility for protein function studies.

mSphere · Aug 21, 2026Peer-reviewedMolecular Biology

Rapid, efficient auxin-inducible protein degradation in Candida pathogens

Kedric L. Milholland, Justin B. Gregor, Smriti Hoda, Soledad Píriz-Antúnez, Encarnación Dueñas‐Santero, Bao Gia Vu, Krishna P. Patel, Scott Moye-Rowley, Carlos R. Vázquez de Aldana, Jaime Correa‐Bordes, Scott D. Briggs, Mark C. Hall

This paper demonstrates the efficient use of auxin-inducible degradation systems in Candida pathogens for rapid protein inactivation.

mSphere · Aug 18, 2023Peer-reviewedMolecular Biology

Reduced Cdc14 phosphatase activity impairs septation, hyphal differentiation and pathogenesis and causes echinocandin hypersensitivity in Candida albicans

Kedric L. Milholland, Ahmed AbdelKhalek, Kortany Maree Baker, Smriti Hoda, Andrew G. DeMarco, Noelle H. Naughton, Angela N Koeberlein, Gabrielle R. Lorenz, Kartikan Anandasothy, Antonio Esperilla-Muñoz, Sanjeev Kumar Narayanan, Jaime Correa‐Bordes, Scott D. Briggs, Mark C. Hall

This paper shows that reducing Cdc14 phosphatase activity in Candida albicans impairs cell wall integrity, septation, hyphal differentiation, and virulence, making it a potential antifungal drug target.

bioRxiv · Sep 29, 2022 · PreprintMolecular Biology