Axel Knebel

Kinase-dead controls, XIC quantification and phosphospecific antibodies establish pSer14, pThr156 and pSer418 as NEK1 autophosphorylation sites; a pThr156 assay shows ALS missense variants differ, with R261C reducing it most.

This study identifies FAF2 as a strong activator of the p97-UN complex and uses its mechanism to design de novo proteins that enhance p97 activity.

This study identifies NEK7-mediated phosphorylation of cortactin as a mechanism by which EML4-ALK V3 enhances cell migration through actin cytoskeleton remodeling.

This study identifies USP5 as the protease responsible for cleaving SDE2UBL, revealing a novel regulatory mechanism for SDE2 function through UBL-directed cleavage.