Darian Stephan Wolff

Generalizable direct protein sequencing with InstaNexus

This paper introduces a workflow for direct protein sequencing using AI-driven methods, validated across diverse proteins to improve accuracy and reduce ambiguity in therapeutic and scientific applications.

Accelerating multi-objective VHH discovery via integrated high-throughput selection and AlphaFold3-guided structure prediction

This paper develops a pipeline combining yeast screening and AlphaFold3 to accelerate discovery of multi-target antibodies, finding that structural predictions improve binding specificity and solubility.

Clearing the whey: Efficient inhibition and removal of chymosin from whey by de novo designed protein-based inhibitors

The paper designs protein-based inhibitors to efficiently inhibit and remove chymosin from whey, enabling sustainable cheese production and enzyme control.

De novo designed pMHC binders facilitate T cell induced killing of cancer cells

This paper designs de novo pMHC binders using generative models to create T cell-based cancer therapeutics with high specificity and minimal cross-reactivity.

Simplified post-assembly plasmid library amplification for increased transformation yields in E. coli and S. cerevisiae

The paper introduces a simplified method for amplifying plasmid DNA libraries using rolling circle amplification and endonuclease treatment to improve transformation efficiency in E. coli and S. cerevisiae.