Author

Holt A. Sakai

Broad InstituteORCID 0000-0002-9338-1484Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Biochemistry· 1 Systems Biology· 1

Signature Assays, Organisms & Methods

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Organism:human· 2 Research Focus:cell type annotation· 1 Research Focus:drug response / drug discovery· 1 Approach:experimental assay· 1 Research Focus:gene regulatory network inference· 1 Approach:machine learning (classical)· 1 Organism:mouse· 1 Assay:proteomics· 1

Frequent Co-Authors

Alexander A. SousaiD· 2 shared Alvin HsuiD· 1 shared Amber N. McElroyiD· 1 shared Ana CristianiD· 1 shared Angus H. LiiD· 1 shared Benjamin J. SteinbeckiD· 1 shared Benjamin P. KleinstiveriD· 1 shared BR Blazar· 1 shared

Activity by Year

2025-2026 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 37 mutual co-author links

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2 shared papersBiochemistry

Alexander A. SousaORCID

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Author profile →

2 papers

Recent·Most cited

Mechanistic machine learning enables interpretable and generalizable prediction of prime editing outcomes

Alvin Hsu, Peter J. Chen, Angus H. Li, Colin F. Hemez, Xin D. Gao, Markus Terrey, C Nelson, Vijay Selvam, Ana Cristian, Amber N. McElroy, Benjamin J. Steinbeck, Gandhar K. Mahadeshwar, Smriti Pandey, Zachary Barsdale, Paul Chen, , David R. Liu

OptiPrime predicts prime editing outcomes with high accuracy, enabling efficient therapeutic applications in human and mouse cells.

bioRxiv · Feb 20, 2026 · PreprintBiochemistry

A scalable proteogenomic framework for dissecting phospho-signaling pathways in primary immune cells

Christian Michel Beusch, Carolyn Morningstar, Marc Semaan, Christopher M. Monaco, Sarah Welbourn, Hailey Swaldi, Jae‐Kyun Ko, Devin Kenney, Alexander A. Sousa, Holt A. Sakai, David Liu, Hisashi Akiyama, Mohsan Saeed, David Ezra Gordon

This paper introduces a proteogenomic framework combining genetic perturbations and proteomics to map phospho-signaling pathways in primary human T cells, validated with inflammatory stimuli.

bioRxiv · Oct 8, 2025 · PreprintSystems Biology