Kimberly J. Vish

The paper optimizes conditions for GTP loading of H-Ras by evaluating factors like time, temperature, concentration, and mutations to enhance nucleotide exchange efficiency.

This study shows the C2 domain enhances Ras GTPase activating protein catalytic activity by interacting with the Ras allosteric lobe, as demonstrated through structural analysis and mutation studies in mice and SynGAP.