The paper develops a 3D-printed stirred bioreactor to improve retinal organoid production by maintaining physiological oxygen levels, enhancing yield and consistency.

Cell Reports Methods · Jun 15, 2026Peer-reviewedBioengineering

Visualization of photoreceptor outer segment renewal using AAV-delivered Dendra2-tagged rhodopsin

Lijing Xie, Dianlei Guo, Xiaoyu Zhang, Yingchun Su, Lei Shi, Shujuan Xu, Rong Ju, Tiansen Li, Chunqiao Liu

This study uses AAV-delivered Dendra2-tagged rhodopsin to track photoreceptor outer segment renewal in mice, showing reduced renewal in a ciliopathy mutant.

bioRxiv · Nov 30, 2025 · PreprintCell Biology

Transcriptome-based molecular staging of human stem cell-derived retinal organoids uncovers accelerated photoreceptor differentiation by 9-cis retinal

Koray Dogan Kaya, Holly Y. Chen, Matthew J. Brooks, Ryan A. Kelley, Hiroko Shimada, Kunio Nagashima, Natalia de Val, Charles T. Drinnan, Linn M. Gieser, Kamil Kruczek, Slaven Erceg, Tiansen Li, Dunja Lukovic, Yogita K. Adlakha, Emily Welby, Anand Swaroop

Comparing retinal organoid transcriptomes against human fetal and adult retina places each culture on a developmental scale; replacing all-trans retinoic acid with 9-cis retinal sped rod differentiation.

bioRxiv · Aug 12, 2019 · PreprintDevelopmental Biology