Josefina Maranzano

T2 relaxometry for myelin water fraction: an ex vivo brain imaging study

This study validates and compares TSE and GRASE techniques for myelin water imaging in postmortem human brains, showing moderate to strong agreement between the sequences.

Spatiotemporal trajectories of formaldehyde fixation effects on quantitative MRI in postmortem human brains

This study quantifies how formaldehyde fixation affects MRI metrics in postmortem human brains over time and provides a framework to correct for these effects.

Neuronal quantification in the primary motor cortex of mouse brains fixed with solutions from human gross anatomy laboratories

This study compares the effects of three fixation solutions on neuronal quantification in mouse primary motor cortex, finding no significant differences between them.

Effects of prolonged fixation on vascular biomarkers in postmortem human brains

This study examines how prolonged formalin fixation affects immunohistochemical and histochemical stains in postmortem human brains, finding decreased staining for specific markers over time.

Immunofluorescence quality of human brain tissue fixed with solutions used in gross anatomy laboratories

The paper compares immunofluorescence quality in human brains fixed with different solutions and finds Sudan Black B effectively reduces autofluorescence.

Histology to MRI registration quality of ex vivo human brain blocks fixed with solutions used in anatomy laboratories

The paper compares MRI-histology registration quality in human brain blocks fixed with different solutions and finds that SSS-fixed blocks require more landmarks for alignment but still achieve sufficient registration quality.

Comparison of T1- and T2-weighted MRI contrasts of ex vivo ex situ brains fixed with solutions used in gross anatomy laboratories

The paper compares T1- and T2-weighted MRI contrasts in brains fixed with different solutions, finding T2w images better suited for structural analysis.

Ex vivo human brain volumetry: validation of magnetic resonance imaging measurements

This paper validates MRI brain volume measurements using ex vivo specimens and finds that SynthSeg provides accurate T1-weighted volumes compared to water displacement.

Differential effects of prolonged post-fixation on immunohistochemical and histochemical staining for postmortem human brains

The paper examines how prolonged post-fixation affects immunohistochemical and histochemical staining in postmortem human brains, finding mixed correlations with staining intensity and limited effects of age and PMI.

The Douglas Bell Canada Brain Bank Post-mortem Brain Imaging Protocol

The paper describes a postmortem MRI protocol for the Douglas-Bell Canada Brain Bank to acquire high-resolution images while preserving tissue for histological analysis.

Comparison of histological procedures and antigenicity of human post-mortem brains fixed with solutions used in gross anatomy laboratories

The paper compares histological quality and antigenicity preservation in human brains fixed with different solutions, finding that AFS-fixed brains show better antigen labeling and tissue quality than NBF or SSS.

Antigenicity is preserved with fixative solutions used in human gross anatomy: A mice brain immunohistochemistry study.

This study compares fixative solutions used in human gross anatomy labs to preserve brain tissue antigenicity in mice, finding that two solutions maintain sufficient antigenicity for immunohistochemistry.

Using Transfer Learning for Automated Microbleed Segmentation

This paper develops an automated microbleed segmentation tool using transfer learning on MRI data, achieving high sensitivity and specificity.

White Matter Hyperintensity Distribution Differences in Aging and Neurodegenerative Disease Cohorts

The paper compares white matter hyperintensity (WMH) volumes across neurodegenerative disease groups and sexes, finding greater WMH burden in cognitively impaired groups and vascular disease subgroups.

Diffusely abnormal white matter converts to T2 lesion volume in the absence of acute inflammation

This paper tracks the conversion of diffusely abnormal white matter to T2 lesions in MS patients, showing it occurs independently of inflammation and gadolinium enhancement.

PhyloBrain atlas: a cortical brain MRI atlas following a phylogenetic approach

The paper introduces a cortical brain MRI atlas divided into regions based on phylogenetic evolution, including five main ROIs and twelve neocortical sub-ROIs.

Proof of concept of a novel ex vivo, in situ method for MRI and histological brain assessment

This paper demonstrates that perfusion-based in situ fixation of ex vivo brains preserves structural integrity and antigenicity, enabling accurate MRI and histological analysis up to 10 months post-mortem.

Conversion of Diffusely Abnormal White Matter to Focal Lesions is Linked to Progression in Secondary Progressive Multiple Sclerosis

The paper segments and tracks white matter lesions in MS patients, finding that transformation of diffuse abnormal white matter to focal lesions correlates with clinical progression.

Automated Separation of Diffusely Abnormal White Matter from Focal White Matter Lesions on MRI in Multiple Sclerosis

This paper automates the separation of focal white matter lesions and diffusely abnormal white matter in MS using MRI intensity thresholds and finds MTR values are lower in focal lesions compared to diffusely abnormal white matter.

White Matter Hyperintensities and Cognitive Decline in de Novo Parkinson’s Disease Patients

This study finds that high baseline white matter hyperintensities (WMHs) in de novo Parkinson’s disease patients are linked to greater cognitive decline and cortical thinning compared to low WMHs or controls.