This study demonstrates that rhodopsin-based voltage indicators can be used for two-photon voltage imaging, achieving high-fidelity action potential detection in vitro and in vivo.

Neuron · Feb 12, 2026Peer-reviewedNeuroscience

High-throughput in vivo synaptic connectivity mapping of neuronal micro-circuits using two-photon holographic optogenetics and compressive sensing

I-Wen Chen, Chung Yuen Chan, Phillip Navarro, Vincent de Sars, Emiliano Ronzitti, Karim G. Oweiss, Dimitrii Tanese, Valentina Emiliani

This paper introduces a high-throughput method for in vivo synaptic connectivity mapping using optogenetics and compressive sensing, achieving rapid and accurate identification of synaptic pairs in mice.

bioRxiv · Sep 11, 2023 · PreprintNeuroscience

Ultrafast Light Targeting for High-Throughput Precise Control of Neuronal Networks

Giulia Faini, Dimitrii Tanese, Clément Molinier, Cécile Telliez, Massilia Hamdani, François G. C. Blot, Christophe Tourain, Vincent de Sars, Filippo Del Bene, Benoı̂t C. Forget, Emiliano Ronzitti, Valentina Emiliani

This paper introduces FLiT, a method for ultra-fast sequential light targeting to enable precise and scalable control of neuronal networks.

Nature Communications · Apr 5, 2023Peer-reviewedNeuroscience

WiChR, a highly potassium selective channelrhodopsin for low-light two-photon neuronal inhibition

Johannes Vierock, Enrico Peter, Christiane Grimm, Andrey Rozenberg, Alejandro G. Castro Scalise, Sandra Augustin, Dimitrii Tanese, Benoı̂t C. Forget, Valentina Emiliani, Oded Béjà, Peter Hegemann

The paper identifies a highly potassium-selective channelrhodopsin (WiChR) that enables efficient neuronal inhibition through two-photon optogenetics under low-light conditions.

bioRxiv · Jul 2, 2022 · PreprintNeuroscience

Evolutionary divergence of locomotion in two related vertebrate species

Gokul Rajan, Julie Lafaye, Giulia Faini, Martín Carbó Tano, Karine Duroure, Dimitrii Tanese, Thomas Panier, Raphaël Candelier, Jörg Henninger, Ralf Britz, Benjamin Judkewitz, Christoph Gebhardt, Valentina Emiliani, Georges Debrégeas, Claire Wyart, Filippo Del Bene

This study compares swimming patterns and neuronal circuits in two closely related fish species to understand how environmental factors drive evolutionary divergence in locomotion.

Cell Reports · Mar 1, 2022Peer-reviewedNeuroscience

High-throughput cellular-resolution synaptic connectivity mapping in vivo with concurrent two-photon optogenetics and volumetric Ca2+ imaging

Christopher McRaven, Dimitrii Tanese, Lixia Zhang, Chao-Tsung Yang, Misha B. Ahrens, Valentina Emiliani, Minoru Koyama

The paper develops a high-throughput method to map synaptic connectivity in zebrafish using two-photon optogenetics and volumetric Ca2+ imaging.

bioRxiv · Feb 21, 2020 · PreprintNeuroscience

Multiplexed temporally focused light shaping for high-resolutionmulti-cell targeting

Nicolò Accanto, Clément Molinier, Dimitrii Tanese, Emiliano Ronzitti, Zachary L. Newman, Claire Wyart, Ehud Y. Isacoff, Eirini I. Papagiakoumou, Valentina Emiliani

The paper introduces a method for high-resolution multi-cell optogenetic targeting using temporally focused light shaping in Drosophila and zebrafish larvae.

Optica · Nov 15, 2018Peer-reviewedNeuroscience

Sub-millisecond optogenetic control of neuronal firing with two-photon holographic photoactivation of Chronos

Emiliano Ronzitti, Rossella Conti, Eirini Papagiakoumou, Dimitrii Tanese, Valeria Zampini, Emmanuelle Chaigneau, Amanda J Foust, Nathan Klapoetke, Ed S Boyden, Valentina Emiliani

This study demonstrates sub-millisecond optogenetic control of neuronal firing using two-photon holographic photoactivation of Chronos, achieving precise and repeated spiking up to 100 Hz.

bioRxiv · Jul 5, 2016Neuroscience