Author

Cilia R. Pothast

Leiden University Medical CenterORCID 0000-0002-9761-3123Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Research Focus:cell type annotation· 2 Approach:experimental assay· 2 Organism:human· 2 Research Focus:cell-cell communication· 1 Assay:CITE-seq· 1 Research Focus:differential expression· 1 Assay:flow cytometry· 1

Frequent Co-Authors

Mirjam H.M. HeemskerkiD· 2 shared Wesley HuismaniD· 2 shared Alicia C. de Kroon· 1 shared Angela F. El HebieshyiD· 1 shared Anna H.E. RoukensiD· 1 shared Anneloes van der Plas - van DuijniD· 1 shared Dicky L. TahaparyiD· 1 shared Ferenc A. ScheereniD· 1 shared

Activity by Year

2024-2025 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 30 mutual co-author links

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2 shared papersImmunology

Mirjam H.M. HeemskerkORCID

Mutual co-authors in cluster

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2 papers

Recent·Most cited

Immuno-functionomics reveals geographical variation and a role for TLR8 in mRNA vaccine responses

Wesley Huisman, Shohreh Azimi, Thi Yen Nhi Nguyen, Alicia C. de Kroon, Karin de Ruiter, Dicky L. Tahapary, Mikhael D. Manurung, Cilia R. Pothast, Yvonne C. M. Kruize, Mirjam H.M. Heemskerk, Taniawati Supali, Leo G. Visser, Anna H.E. Roukens, Maria Yazdanbakhsh, Simon P. Jochems

The study uses spectral flow cytometry to analyze immune function across populations, revealing geographical differences and TLR8's role in mRNA vaccine responses.

iScience · Oct 25, 2025Peer-reviewedImmunology

Temperature-based MHC class-I multimer peptide exchange for human HLA-A, B and C.

Cilia R. Pothast, Ian Derksen, Anneloes van der Plas - van Duijn, Angela F. El Hebieshy, Wesley Huisman, Kees L. M. C. Franken, Jacques Neefjes, Jolien J. Luimstra, Marieke Griffioen, Michel G.D. Kester, Maarten H. Vermeer, Marcus Östholm, Sine Reker Hadrup, Mirjam H.M. Heemskerk, Ferenc A. Scheeren

The paper introduces a temperature-based peptide exchange method for loading different peptides into MHC-I multimers for HLA-A, B, and C alleles, enabling high-throughput T cell response analysis.

bioRxiv · Dec 23, 2024 · PreprintImmunology