Masayuki Onishi

Dual-targeted NADK2 Links Mitochondrial Redox Homeostasis to Carbon Partitioning and Heterotrophic Growth in Chlamydomonas reinhardtii

This study shows that CreNADK2 localizes to both chloroplasts and mitochondria in Chlamydomonas reinhardtii, and its absence impairs mitochondrial NADP(H) synthesis, TCA cycle function, and heterotrophic growth.

Testing Reversibility of Endosymbiotic Gene Transfer between Chloroplast and Nucleus

This study reverses endosymbiotic gene transfer by moving nuclear-encoded chloroplast proteins back to the chloroplast genome in Chlamydomonas reinhardtii, showing proper localization and function.

Tubulin Acetylation Deficiency Promotes Axonemal Turnover and Increases Cytoplasmic Microtubules

The paper shows that tubulin acetylation deficiency increases axonemal turnover and cytoplasmic microtubule dynamics without affecting flagellar length or cell growth.

Rapid adaptive evolution of microbial thermal performance curves

This study shows how genetic and environmental factors shape microbial thermal performance curves and their evolution under temperature changes.

Septin-mediated coupling of protein import and division during chloroplast evolution

This study identifies SEP1, a septin in Chlamydomonas reinhardtii, as a coordinator of chloroplast protein import and division, showing its evolutionary conservation and functional interaction with TOC GTPases.

Mitotic entry is controlled by the plant-specific phosphatase BSL1 and cyclin-dependent kinase B

This study identifies BSL1 as a plant-specific phosphatase that dephosphorylates CDKB1 to promote mitotic entry by counteracting phosphorylation at T14/Y15 residues.

Imaging-based screen identifies novel natural compounds that perturb cell and chloroplast division in Chlamydomonas reinhardtii

This study screens natural compounds to identify those affecting cell and chloroplast division in Chlamydomonas reinhardtii, finding that curcumin disrupts chloroplast division without affecting microtubule structures.

The Evolutionary Origins and Ancestral Features of Septins

This study investigates the evolutionary origins and ancestral features of septins by analyzing their phylogenetic distribution and structural evolution across eukaryotes.

Alternative proteoforms and proteoform-dependent assemblies in humans and plants

The paper identifies proteoform truncations in human and plant proteomes and describes their functional implications and evolutionary conservation.

Temperature alone is not enough: food-web context determines evolutionary responses to warming

This study shows that food-web context, not just temperature, determines prey evolutionary responses to warming through predator interactions and plasticity.

Systematic characterization of gene function in a photosynthetic organism

The paper systematically characterizes gene function in a photosynthetic organism by analyzing mutant phenotypes across diverse conditions and linking them to functional pathways.