James Chuang

Cooperative assembly confers regulatory specificity and long-term genetic circuit stability

This study shows that cooperative self-assembly of transcriptional regulators can improve regulatory specificity and long-term stability of synthetic gene circuits in yeast.

Suppressor mutations that make the essential transcription factor Spn1/Iws1 dispensable in Saccharomyces cerevisiae

The paper identifies suppressor mutations in yeast that allow survival without the essential transcription factor Spn1/Iws1 by revealing multiple pathways that compensate for its loss.

Essential histone chaperones collaborate to regulate transcription and chromatin integrity

This study identifies a network of histone chaperones and elongation factors that coordinate transcriptional accuracy and chromatin structure through their physical interactions.

The conserved elongation factor Spn1 is required for normal transcription, histone modifications, and splicing in Saccharomyces cerevisiae

This study shows that Spn1 is essential for normal mRNA levels, splicing of ribosomal protein transcripts, and histone modifications in yeast.

Spt6 is required for the fidelity of promoter selection

This study shows Spt6 represses transcription initiation at thousands of intragenic promoters and regulates initiation at most genic promoters, proposing a model of initiation-site competition.