Manoj Kumar

This study uses FnCas9 to generate fluorescently labeled hESC lines for tracking and sorting neural lineages in 2D cultures and 3D cortical organoids.

CriSNPr provides a webserver for designing and querying gRNAs for CRISPR-based diagnostics using multiple Cas systems and includes a curated database of gRNAs for human and SARS-CoV-2 variants.

FELUDA uses FnCas9 for rapid, accurate nucleobase detection and is implemented in a point-of-care diagnostic tool for SARS-CoV-2.

This paper demonstrates that Francisella novicida Cas9 (FnCas9) has high specificity and low off-target editing, enabling precise genome editing in mammalian cells.