Debojyoti Chakraborty

This paper presents a scalable workflow for producing high-purity circular single-stranded DNA using an M13 phagemid system, achieving yields of 120-195 µg from 300 mL culture.

StickForStats automatically validates statistical assumptions in computational biology analyses, improving reproducibility by default-checking assumptions and rerouting to nonparametric methods when violations occur.

This study generates iPSC models of MLC by reprogramming patient-derived cells and gene editing, revealing disease-specific vacuolation in astrocytes and differentially expressed genes related to neurological disorders.

This study develops a CRISPR-based diagnostic method to rapidly and reliably detect haemoglobin variants S, C, D, and E in saliva and urine samples.

This study uses FnCas9 to generate fluorescently labeled hESC lines for tracking and sorting neural lineages in 2D cultures and 3D cortical organoids.

CriSNPr provides a webserver for designing and querying gRNAs for CRISPR-based diagnostics using multiple Cas systems and includes a curated database of gRNAs for human and SARS-CoV-2 variants.

This study identifies TOBF1 as a regulator of alternative splicing in mouse embryonic stem cells, showing its role in maintaining stem cell identity through co-transcriptional mechanisms.

FELUDA uses FnCas9 for rapid, accurate nucleobase detection and is implemented in a point-of-care diagnostic tool for SARS-CoV-2.

This paper demonstrates that Francisella novicida Cas9 (FnCas9) has high specificity and low off-target editing, enabling precise genome editing in mammalian cells.