Constantinos Patinios

Helicase family proteins promote antiviral ADP-ribosylation of DNA purines

The paper identifies a new DarTG subfamily in E. coli that uses a helicase protein to promote ADP-ribosylation of DNA purines, expanding the known functions of helicases in bacterial immunity.

RNA editing and trans-splicing with reprogrammed tracrRNAs

This paper demonstrates that reprogrammed tracrRNAs can enable RNA base editing and trans-splicing through Cas9 recruitment, offering a versatile method for RNA manipulation.

Targeted DNA nicking enables efficient, single-step and counterselection-free editing of bacteriophage genomes

This paper introduces a method for efficient, single-step genome editing of bacteriophages using targeted DNA nicking, achieving high recombination rates without reducing phage titers.

Deletion of the moe A gene in Flavobacterium IR1 drives structural color shift from green to blue and alters polysaccharide metabolism

The paper shows that deleting the moeA gene in Flavobacterium IR1 changes structural color from green to blue and affects polysaccharide metabolism.

Structure of an oxygen-induced tubular nanocompartment in Pyrococcus furiosus

The paper identifies oxygen-induced tubular nanocompartments in Pyrococcus furiosus using proteomics and cryo-EM, revealing their structure and role in ROS protection.

Streamlined and efficient genome editing in Cupriavidus necator H16 using an optimised SIBR-Cas system

This study optimizes a genome editing system for Cupriavidus necator H16, achieving high editing efficiency using an improved SIBR-Cas system.

Targeted DNA ADP-ribosylation triggers templated repair in bacteria and base mutagenesis in eukaryotes

This paper introduces append editing, a method that uses ADP-ribosylation to achieve distinct genome editing outcomes in bacteria and eukaryotes.

A cell-free transcription-translation pipeline for recreating methylation patterns boosts DNA transformation in bacteria

IMPRINT uses cell-free transcription-translation to methylate DNA, bypassing bacterial restriction and improving transformation efficiency in diverse bacteria.