Thomas Alexander Hooven

This study develops a CRISPR interference system in a Streptococcus agalactiae ST-17 strain to enable tunable gene knockdown, demonstrating its utility in studying virulence factors at the blood-brain barrier.

This paper develops a Cas12a-based toolkit for efficient genomic editing and CRISPRi in Group B Streptococcus, enabling targeted gene modifications and inducible gene silencing with minimal off-target effects.

This study uses CRISPRi to identify Sip as a key surface protein in GBS that modulates host interactions and pathogenesis.