Dan Liang

The paper functionally characterizes enhancers in totipotent-like cells using reporter assays and integrates with epigenomic and transcriptomic data to show their role in ZGA gene transcription.

This study shows β-catenin drives apical-basal polarization and TE lineage commitment in mouse embryos in vitro and in vivo.

This paper develops an antibody-free lipid nanoparticle system for in vivo mRNA delivery to human HSCs, enabling efficient base editing of HBG to treat blood disorders like beta-thalassemia and sickle cell disease.

This study demonstrates that CRISPR/Cas9-mediated gene conversion can efficiently repair genetic mutations in hematopoietic stem cells without requiring exogenous DNA templates.

This study shows that gene conversion and NHEJ are major DNA repair mechanisms in human embryos, with gene conversion potentially useful for gene correction but also causing extensive loss of heterozygosity.