Yuxuan Wu

Orthogonal and Robust Analytics Enable Reproducible and Scalable Manufacturing of High Purity Extracellular Vesicles Derived from Mesenchymal Stromal Cells

This paper develops an analytical toolbox and scalable downstream processing workflow for high purity extracellular vesicles from mesenchymal stromal cells, using orthogonal methods to improve reproducibility and purity.

In vivo genome editing of human hematopoietic stem cells for treatment of blood disorders by mRNA delivery

This paper develops an antibody-free lipid nanoparticle system for in vivo mRNA delivery to human HSCs, enabling efficient base editing of HBG to treat blood disorders like beta-thalassemia and sickle cell disease.

Sequential Amino Acid Mutagenesis-Driven De Novo Evolution of Adenine Deaminases Enables Efficient In Vivo Base Editing in Primate

This paper develops hpABE5.20, a base editor with improved precision and reduced off-target effects through sequential amino acid mutagenesis and AI-based structural prediction.

Efficient repair of human homozygous genetic mutation by CRISPR/Cas9 mediated interlocus gene conversion

This study demonstrates that CRISPR/Cas9-mediated gene conversion can efficiently repair genetic mutations in hematopoietic stem cells without requiring exogenous DNA templates.