Calina A. Glynn

Femtosecond pulsed laser ablation for in-tissue electron cryo-tomography

This paper demonstrates that cryogenic femtosecond pulsed laser ablation can efficiently prepare vitreous specimens of mammalian brain tissue for cryo-ET, enabling routine in situ cell biology of multicellular samples.

Charting the molecular landscape of neuronal organisation within the hippocampus using cryo electron tomography

Xenon plasma FIB cryo lift-out from high-pressure-frozen mouse hippocampal slices up to 200 µm thick enables fluorescence-guided cryoET of CA1 synapses and apical dendrite cytoskeleton.

Reduction of SEM charging artefacts in native cryogenic biological samples.

This paper reduces SEM charging artefacts in cryogenic biological samples using interleaved scanning, enabling clearer visualization of previously masked structures.

Tau seeding and spreading in vivo is supported by both AD-derived fibrillar and oligomeric tau

The paper compares the bioactivity of fibrillar and oligomeric tau forms in Alzheimer's disease, finding HMW tau more potent in glial activation and tau propagation.