Mohammad Sharifian Gh.

This study identifies N-104, a cleavage-potentiated form of lacritin, as a bactericidal agent that alters the transcriptome of P. aeruginosa PA14, reducing virulence, fitness, metabolism, and stress response while increasing efflux and anaerobic respiration.

The paper identifies a non-lytic bactericidal mechanism in P. aeruginosa by targeting multiple transporters and transcription, leading to respiratory suppression and bacterial death.

This paper introduces a luciferase-based assay to measure antibiotic permeability into the cytosol of Gram-negative bacteria and demonstrates its ability to track intracellular accumulation in real time.