Marcos M. Pires

This study shows lipid conjugates have different effects on cytosolic accumulation in E. coli and mammalian cells, highlighting context-dependent permeation strategies.

This study investigates the structure-activity relationship of griselimycin, identifying key amino acids and modifications that enhance its antitubercular activity.

This paper evaluates peptidomimetic modifications of MHC-I epitopes to improve antigen design, finding position-dependent tolerance and non-additive effects on immunogenicity and pharmacokinetics.

This study identifies LdtB as essential for Mycobacterium tuberculosis acid resistance by linking peptidoglycan cross-linking to intrabacterial pH homeostasis and antibiotic susceptibility.

This paper develops a high-throughput peptide-based assay to profile L,D-transpeptidase activity and inhibitors in mycobacteria, showing potent inhibition by (carba)penems.

This study develops a flow cytometry method to measure biomolecular condensate formation, colocalization, and exchange dynamics, revealing that condensate aging reduces molecular dynamics.

The paper identifies benzimidazole compounds, including nocodazole, as NOD2 agonists with potential therapeutic benefits over existing MDP-based agonists.

The paper shows that chemical modifications of MHC-I epitopes affect T cell recognition, demonstrating that antigenic identity depends on both sequence and chemical history.

This paper introduces D-CLAP, a split-luciferin assay to measure real-time cytosolic accumulation of compounds in live mycobacteria, showing that modifying carboxylates increases accumulation and antimycobacterial activity.

The paper identifies Hsp90 inhibitors as MHC-I inducers and develops novel compounds using click chemistry to enhance T cell activation and reduce toxicity.

The paper evaluates how structural modifications like macrocyclization and N-alkylation affect peptide accumulation in Mycobacterium tuberculosis using a novel assay, showing macrocyclization generally enhances accumulation while N-alkylation has context-dependent effects.

This paper shows that the mycomembrane of Mycobacterium tuberculosis and M. smegmatis differentially and heterogeneously restricts antibiotic permeation using the PAC-MAN method.

The paper introduces the CHAMP assay to measure cytosolic drug accumulation in mammalian cells, showing how molecular structure affects penetration.

The paper identifies a non-lytic bactericidal mechanism in P. aeruginosa by targeting multiple transporters and transcription, leading to respiratory suppression and bacterial death.

The paper identifies chemical features affecting mycomembrane permeation and antitubercular activity using a bioorthogonal assay and machine learning.

This study identifies a new family of LDTs in C. difficile that catalyze 3-3 peptidoglycan crosslinking, essential for viability and distinct from previously known YkuD domain LDTs.