Jay D. Keasling

The paper develops a highly sensitive biosensor for caprolactam and valerolactam from Pseudomonas putida, capable of detecting product titers above background at ~6 g/L.

This paper describes a method to evolve and functionalize biosensors in yeast by optimizing ligand specificity and transfer functions through directed evolution.

The paper characterizes two glutarate-sensing transcription factors in Pseudomonas putida and develops biosensors to detect glutarate flux for metabolic engineering.

This study identifies and disrupts genes in Pseudomonas putida that cause unwanted metabolism of valerolactam, increasing its production titer.

This paper introduces a yeast platform for producing bacterial aromatic polyketides using a synthetic polyketide synthase system.

The paper develops a synthetic selection system in yeast to enrich for high-producing muconic acid variants by coupling production to cell fitness through a pH-tunable sensor-selector.