Basheer Becerra

This paper introduces a method for targeted genomic integration and rearrangement in human cells using CRISPR-targeted dual flap synthesis, enabling DNA sequence assembly without requiring double-stranded DNA donors or cell cycle progression.

CRISPR-CLEAR maps regulatory elements at nucleotide resolution by directly assessing genotype-phenotype effects through targeted sequencing of CRISPR-introduced alleles.

The paper introduces ProBound, a machine learning framework that quantifies biophysical parameters of protein-ligand interactions using NGS data, and couples it with Kd-seq to determine absolute affinities.