Gue‐Ho Hwang

This study identifies IGF2BP1 as a direct and indirect regulator of fetal globin transcripts through m6A modification and interaction with HIC2 and BCL11A.

This study shows that base editing in human embryos produces efficient on-target changes without chromosomal abnormalities or large deletions, unlike CRISPR/Cas9-induced DSBs.

The paper introduces a nanopore sequencing method with Cas9 targeting and an analysis algorithm to detect STR expansions, identifying pathogenic repeats in cerebellar ataxia patients.

CRISPRLungo is a computational pipeline for analyzing long-read CRISPR experiments, improving accuracy in detecting structural variants and small indels through UMI-based error correction and benchmarking.

This paper introduces a method for targeted genomic integration and rearrangement in human cells using CRISPR-targeted dual flap synthesis, enabling DNA sequence assembly without requiring double-stranded DNA donors or cell cycle progression.

The paper shows that bystander base editing with ABE8e impairs visual function restoration in LCA model mice by causing structural and functional disruptions in RPE65.

The paper identifies mechanisms by which CRISPR, base editors, and prime editors cause large DNA deletions and proposes strategies to mitigate them.

This paper tests prime editing in human pluripotent stem cells, showing it can induce various genetic modifications and correct disease mutations without off-target effects from the reverse transcriptase domain.

CRISPR-pass uses adenine base editors to bypass nonsense mutations in patient-derived fibroblasts, showing potential for treating genetic disorders.

The paper introduces two web tools, BE-Designer and BE-Analyzer, for designing CRISPR base editor targets and analyzing next-generation sequencing data.