Naphat Satapoomin

This study shows extracellular Orientia tsutsugamushi contributes to systemic dissemination and that gentamicin reduces bacterial growth in a murine model.

This study compares seven strains of Orientia tsutsugamushi to identify virulence factors, finding no single gene or pattern predicts virulence, but highlighting complex interactions among effector proteins.

The paper shows that gene cassettes in an integron, amplified by IS26, confer resistance to amikacin and piperacillin/tazobactam, often leaving meropenem as the last-resort antibiotic.

The paper identifies specific amino acid substitutions in NfsA and NfsB that reduce enzyme activity and cause nitrofurantoin resistance in E. coli, but finds that WGS alone cannot reliably predict resistance due to functional silent mutations and downregulated gene expression.

Hound maps genotype to phenotype in bacterial genomes by de novo assembly, gene detection, and mutation reporting, demonstrating its utility in predicting antibiotic resistance.