Cayetano Pleguezuelos‐Manzano

This study compares hiPSC-derived intestinal epithelial cells cultured in organoids, Transwells, and intestine-on-chip systems, showing that the latter promotes stronger gene expression related to nutrient and drug metabolism and a more mature state.

The paper introduces PTATO, a bioinformatic workflow for detecting somatic mutations in single-cell WGS data using PTA, achieving high sensitivity for base substitutions and indels.

The paper uses CRISPR/Cas9 to generate mutant intestinal organoids and identifies TMPRSS2 as a critical host factor for multiple coronaviruses.

This study evaluates prime editing for modeling cancer mutations and repairing CFTR in intestinal organoids, showing its potential but also highlighting challenges in efficiency and off-target effects.