Mart Matthias Lamers

The paper develops an integration-free CRISPR platform to study host dependencies of respiratory viruses in human nasal epithelium, showing ACE2 and TMPRSS2 are critical for SARS-CoV-2 and hCoV-NL63 infection.

The paper uses nanopore sequencing to show herpesvirus mRNAs have longer poly(A) tails than cellular or other viral transcripts, suggesting a unique mechanism for enhancing gene expression.

This study investigates NLRP3 inflammasome activation in human keratinocytes, showing it is induced by IFN-gamma but not LPS, and that specific activators like Hla target NLRP3 while nigericin activates both NLRP1 and NLRP3.

The paper uses CRISPR/Cas9 to generate mutant intestinal organoids and identifies TMPRSS2 as a critical host factor for multiple coronaviruses.