Ran Brosh

This study identifies DeSI1 as a cyclin D-CDK4/6 substrate whose phosphorylation regulates membrane and mitochondrial protein supply during proliferation.

The paper introduces an improved plasmid toolkit to streamline the mSwAP-In method for large-scale genome writing in mammalian cells.

This paper introduces a method to deliver synthetic haplotypes containing T2D GWAS variants to hiPSCs to study their functional impact on differentiation.

This study introduces a humanized mouse model of XDP with SVA_F insertion in TAF1, revealing myelin loss and oligodendrocyte dysfunction as key pathogenic features.

The paper genomically rewrote a mouse Taf1 allele with a human XDP patient-derived TAF1 allele, revealing that intronic SVA retrotransposons reduce TAF1 levels and cause neurodegeneration.

The paper identifies context-dependent enhancer synergy in mouse embryonic stem cells, showing that enhancers can significantly boost neighboring DHS activity and that this synergy depends on distance and specific transcription factor sequences.

The paper introduces an automated workflow for high-throughput assembly of large DNA libraries using yeast-based methods, achieving high success rates in assembling diverse DNA constructs.

The paper investigates how genomic context affects enhancer function by analyzing the murine Igf2/H19 locus and shows that regulatory elements depend on their native surroundings.

This paper introduces mSwAP-In, a method for efficient genome rewriting in mouse embryonic stem cells, demonstrating its use for Trp53 and ACE2 locus modifications to create more accurate disease models.

The paper uses synthetic regulatory genomics to study Sox2 expression in mESCs, finding that some enhancer regions are context-dependent and can enhance core regulatory elements.

This study demonstrates that a circular human artificial chromosome can be transmitted through three generations of mice without health or reproductive issues.

The paper introduces a reversed synthetic locus into yeast and mouse genomes to study default chromatin states, finding widespread transcription in yeast but not in mouse.

This paper introduces CREEPY, a method for efficiently editing large episomal DNA constructs in yeast, demonstrating its application to a mouse Sox2-harboring episome.

The paper investigates how genomic context affects CTCF insulator function by analyzing reporter activity across various genomic configurations.

The paper uses scRNAseq data from mouse and human to identify candidate genes for glial fate specification and optimize astrocyte differentiation protocols.

The paper uses synthetic genomic reconstitution to study Hox cluster regulation, finding that distal enhancers are required for full transcriptional output while chromatin remodeling can occur without them.

This study shows PRDM14 is essential for human PGC fate, contradicting previous findings, and reveals divergent regulatory mechanisms between human and mouse PGCs using inducible degrons.

The paper describes a pipeline for de novo assembly, delivery, and expression of a 101 kb human gene in mouse cells, demonstrating functional evaluation of a full-length gene.