The paper introduces a split-GFP graphene grid platform for selective protein capture in cryo-EM, demonstrating improved enrichment and a 2.58 Å cryoEM reconstruction of GFP11-tagged apoferritin.

bioRxiv · Sep 14, 2026 · PreprintBiochemistry

Eco-Microbiology: Discovering Biochemical Enhancers of PET Biodegradation by Piscinibacter sakaiensis

Felipe-Andrés Piedra, Miguel A. Salazar, Sara Abouelniaj, Raayed Rahman, Justin C. Clark, Yimo Han, Zhao Wang, Anthony William Maresso

This paper identifies biochemical enhancers of PET biodegradation by Piscinibacter sakaiensis using iterative screening and biodegradation assays.

Applied and Environmental Microbiology · Feb 24, 2025Peer-reviewedMicrobiology

Plural molecular and cellular mechanisms of pore domain KCNQ2 encephalopathy

Timothy J Abreo, Emma C. Thompson, Anuraag Madabushi, Heun Soh, Nissi Varghese, Carlos G. Vanoye, Kristen Springer, Kristen Springer, Jim Johnson, Scotty Sims, Zhigang Ji, Ana G. Chavez, Miranda J. Jankovic, Bereket Habte, Aamir R. Zuberi, , Edward C. Cooper

The paper investigates the molecular and cellular mechanisms of KCNQ2 encephalopathy through structural analysis, functional assays, and an animal model, revealing multiple pathogenic effects of the G256W variant.

eLife · Jan 6, 2025Peer-reviewedNeuroscience

TomoScore: A Neural Network Approach for Quality Assessment of Cellular cryo-ET

Xuqian Tan, Xueting Zhou, Ethan Boniuk, Anisha Abraham, Zhili Yu, Valerie Dalton, Steven J. Ludtke, Zhao Wang

TomoScore assesses cryo-ET tomogram quality for cellular annotation using a deep learning model, identifying optimal electron dose ranges to improve data processing efficiency.

bioRxiv · Nov 6, 2024 · PreprintBiophysics