Ron Kelley

This study investigates how ion species and milling voltage affect cryo-pFIB-SEM image quality, showing that optimal settings depend on specimen composition.

Automated cryogenic plasma-focused ion beam milling and cryo-electron tomography of Chlamydomonas reinhardtii yield 1,829 annotated cellular tomograms and sub-nanometer in situ structures of six macromolecular complexes.

The paper uses cryo-electron tomography to visualize mitochondrial respiratory complexes in Chlamydomonas reinhardtii, revealing their organization into distinct membrane domains and a respirasome structure with cytochrome c binding.

This paper introduces a method using metallic platinum sputtering to improve subtomogram averages, enabling sub-5 Angstrom resolution of yeast ribosomes with fewer particles.