Abhay Kotecha

This study investigates how ion species and milling voltage affect cryo-pFIB-SEM image quality, showing that optimal settings depend on specimen composition.

The paper analyzes depth-dependent structural damage caused by cryo-FIB milling using apoferritin as a reporter, showing damage varies with ion type and molecular target.

Automated cryogenic plasma-focused ion beam milling and cryo-electron tomography of Chlamydomonas reinhardtii yield 1,829 annotated cellular tomograms and sub-nanometer in situ structures of six macromolecular complexes.

This paper demonstrates that a 100 keV cryo-TEM with advanced components can achieve sub-3 Å resolution structures of various proteins and viruses.

The paper uses cryo-electron tomography to visualize mitochondrial respiratory complexes in Chlamydomonas reinhardtii, revealing their organization into distinct membrane domains and a respirasome structure with cytochrome c binding.

The paper reports cryo-EM structures of tau filaments formed in SH-SY5Y cells seeded with brain extracts from Alzheimer's and corticobasal degeneration cases, showing some structural templating.

The paper determines high-resolution structures of CDK-activating kinase using cryo-EM, revealing inhibitor interactions and providing a framework for structure-based drug design.

The paper identifies a SNCA mutation causing juvenile-onset synucleinopathy and describes the structural features of α-synuclein filaments associated with the disease.

The paper reports cryo-EM structures of α-synuclein filaments from Parkinson's disease, PD dementia, and dementia with Lewy bodies, showing they are composed of a single protofilament distinct from those in multiple system atrophy.

This paper introduces a method using metallic platinum sputtering to improve subtomogram averages, enabling sub-5 Angstrom resolution of yeast ribosomes with fewer particles.

This study identifies age-dependent formation of TMEM106B amyloid filaments in human brains using cryo-EM, showing they appear in older individuals but not younger ones.

The paper evaluates the performance of new cryo-EM hardware for cryo-ET and subtomogram averaging using EIAV virus-like particles, showing comparable resolution and improved reconstructions with advanced processing methods.

This study reports in vitro assembly of recombinant tau into filaments structurally similar to those in Alzheimer's disease and chronic traumatic encephalopathy using cryo-EM.

Cryo-EM shows brain-derived Aβ42 filaments adopt two related S-shaped protofilament folds: Type I dominates sporadic Alzheimer's, Type II familial Alzheimer's, other disorders and App^NL-F knock-in mice.

Cryo-EM structures sort four-repeat tauopathies into three-layered (PSP, GGT, LNT) and four-layered (CBD, AGD, ARTAG, MAPT intron 10 mutations) tau folds, grounding a hierarchical structure-based classification.