bioRxivMolecular BiologyPreprint
Optimizing Cryo-FIB Lamellas for sub-5 Angstrom in situ Structural Biology
Sagar KhavnekariD, Veronika VrbovskáiD, Magda ZaoralováiD, Ron KelleyiD, Florian BeckiD, Sven KlumpeiD, Abhay KotechaiD, Jürgen M. PlitzkoiD, Philipp S. ErdmanniD
Abstract
We here present a method based on metallic platinum sputtering that can substantially enhance the quality of subtomogram averages from lamellas and thereby reduce the number of particles needed for high-resolution subtomogram averaging. We provide evidence for the physical background of this improvement and demonstrate its usefulness by producing sub-5[A] ribosome averages from yeast.
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