Alicia A. Bicknell

mRNAfold improves mRNA design by co-optimizing stability, local structure, and codon choice, showing better in-solution stability and complex effects on protein production.

The paper shows that mRNA decay during T cell activation occurs through translation-dependent and independent pathways, with ribosome density determining the pathway used.

The paper uses EJC RIPiT-Seq to quantify pre-translational mRNPs and reveals higher flux through AS-NMD pathways than RNA-Seq captures.