Huan Chen

The paper evaluates DNA impurity reduction strategies in rAAV manufacturing, finding that TelN/TelROL and I-SceI reduce plasmid backbone DNA to 20-40%, CRISPR/Cas9 to 10-20%, and Cre/LoxP eliminates it without affecting vector titers.

This study shows that using tissue-specific AAV variants and local delivery reduces hepatic toxicity while maintaining therapeutic efficacy in gene therapy.

The paper develops AAV.eM, a muscle-specific rAAV capsid with reduced off-target transduction, showing efficacy in Duchenne Muscular Dystrophy models and favorable safety in mice and NHPs.

M2IA is a web server that integrates microbiome and metabolome data to identify pathways linked to obesity using TwinsUK cohort data.