This paper introduces a step-emulsion platform for scalable microgel fabrication that is robust, user-agnostic, and compatible with various materials and viscosities.

bioRxiv · May 21, 2026 · PreprintBioengineering

Stepwise Stiffening/Softening of and Cell Recovery from Reversibly Formulated Hydrogel Double Networks

Irina Kopyeva, Ethan C. Goldner, Jack W. Hoye, Shiyu Yang, Mary C. Regier, John C Bradford, Kaitlyn R. Vera, Ross C. Bretherton, Jennifer L. Robinson, Cole A. DeForest

This paper introduces a hydrogel platform with reversible stiffness tuning and cell recovery capabilities to study mechanobiological effects on cell behavior and transcriptome responses.

Advanced Materials · Sep 6, 2024Peer-reviewedBioengineering

Grayscale 4D Biomaterial Customization at High Resolution and Scale

Ivan Batalov, Jeremy R. Filteau, Ryan M. Francis, Georg Jaindl, Luke Orr, Teresa L. Rapp, Shiyu Yang, Jordan A. Filteau, Weisi Xie, Ross C. Bretherton, Adam K. Glaser, Jonathan T.C. Liu, Kelly R. Stevens, Cole A. DeForest

This paper introduces GIZMO, a method to create 3D grayscale hydrogel patterns for more realistic tissue engineering.

bioRxiv · Jan 31, 2024 · PreprintBioengineering