Linda Warfield

The paper maps DNA binding and regulatory targets for nearly all yeast transcription factors, revealing that TF binding does not always correlate with gene regulation and many TFs have dual functions.

The paper investigates the genome-wide roles of yeast Mediator (MED) by depleting its activator-binding domain and finds that MED is directly recruited to most promoters independently of enhancers and activators.

The paper shows that Gal4 and Gcn4 use a sequence-independent 'fuzzy' binding mechanism to interact with Med15, contrasting with their structured interactions with Gal80.

The paper screens random peptides to identify transcription activation domains and develops a deep learning model to predict their function and characteristics.

The paper identifies two gene classes regulated by TFIID and SAGA in yeast, showing functional redundancy for some genes and distinct dependency for others.

The paper examines the properties of two yeast transcription activation domains, Met4 and Ino2, showing they interact with Med15 in a heterogeneous manner similar to Gcn4.

The paper shows that transcription activators form a dynamic fuzzy complex with coactivators through multiple low-affinity interactions, enabling specificity and flexibility in gene regulation.