Luca Sperotto

The paper uses structural biology and computational methods to design MSI-1 mutants that alter RNA binding selectivity, validated by NMR and SPR experiments.

The paper identifies a splicing mechanism in human short introns involving RBM17 and SAP30BP, showing their interaction promotes splicing through a UHM-ULM binding and recruitment to phosphorylated SF3B1.

The paper identifies SPF45/RBM17 as essential for splicing a subset of human short introns, showing it competes with U2AF65 for binding to SF3B1, while U2AF is not required for this process.