In vivo Stability and Biodistribution of Liposome Coated with SlpB from Levilactobacillus brevis

SlpB from Levilactobacillus brevis offers a solution to stabilise liposome in gastrointestinal tract, and to target intestinal APCs in Peyers patches, rendering it a powerful tool for oral delivery of drugs, and to yield the benefits provided by oral delivery. However, the stability of SlpB-coated liposome (SlpB-LP) and its distribution in tissues were not characterized. In this study, we have demonstrated that SlpB-coating could improve the stability of liposome in gastrointestinal tract, and facilitate specific uptake of liposome into Peyers patches, but not intestinal, nor intestinal mucosa. Furthermore, we have shown that uptake of SlpB-LP into Peyers patches enhanced bioavailability of drugs, which have resulted in 427.65-fold increase in bioavailability and at least 2.41-fold decrease in retention of fluorophore in liver where drug metabolism takes places, to a degree which approximate control group. In conclusion, this study shows that SlpB could increase stability of liposome in gastrointestinal tract, increase specific uptake of liposome into Peyers patches, and improve bioavailability. O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=196 SRC="FIGDIR/small/533723v1_ufig1.gif" ALT="Figure 1"> View larger version (69K): org.highwire.dtl.DTLVardef@ed508aorg.highwire.dtl.DTLVardef@42cef8org.highwire.dtl.DTLVardef@2219a9org.highwire.dtl.DTLVardef@c2e3dd_HPS_FORMAT_FIGEXP M_FIG C_FIG