bioRxivNeurosciencePreprint
Shadow imaging for panoptical visualisation of living brain tissue
Yulia DembitskayaiD, Andrew K. J. BoyceiD, Agata IdziakiD, Atefeh Pourkhalili LangeroudiiD, Guillaume Le BourdellèsiD, Jordan Girard, Misa ArizonoiD, Mathieu G. DucrosiD, Marie Sato-Fitoussi, Amaia Otxoa-de-AmezagaiD, Kristell Oizel, Stéphane BanceliniD, Luc MercieriD, Thomas PfeifferiD, Roger J. ThompsoniD, Sun Kwang KimiD, Andréas BikfalviiD, U. Valentin NägerliD
Abstract
Progress in neuroscience research hinges on technical advances in visualizing living brain tissue with high fidelity and facility. Current neuroanatomical imaging approaches either require tissue fixation, do not have cellular resolution or only give a fragmented view. Here, we show how regular light microscopy together with fluorescence labeling of the interstitial fluid in the extracellular space provide comprehensive optical access in real-time to the anatomical complexity and dynamics of living brain tissue.
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