Rab10 inactivation promotes AMPAR trafficking and spine enlargement during long-term potentiation

Jie Wang; Jun Nishiyama; Paula Parra-Bueno; Elwy Okaz; Goksu Oz; Xiaodan Liu; Tetsuya Watabe; Irena Suponitsky-Kroyter; Timothy E. McGraw; Erzsebet M. Szatmari; Ryohei Yasuda

doi:10.7554/elife.103879.3

NeuroscienceeLifeSep 23, 2025

Peer-reviewed journal article

Rab10 inactivation promotes AMPAR trafficking and spine enlargement during long-term potentiation

Jie Wang iD, Jun Nishiyama iD, Paula Parra-Bueno iD, Elwy Okaz, Goksu Oz iD, Xiaodan Liu iD, Tetsuya Watabe iD, Irena Suponitsky-Kroyter, Timothy E. McGraw iD, Erzsebet M. Szatmari iD, Ryohei Yasuda iD

Abstract

Rab-dependent membrane trafficking is critical for changing the structure and function of dendritic spines during synaptic plasticity. Here, we developed highly sensitive sensors to monitor Rab protein activity in single dendritic spines undergoing structural long-term potentiation (sLTP) in rodent organotypic hippocampal slices. During sLTP, Rab10 was persistently inactivated (>30 min) in the stimulated spines, whereas Rab4 was transiently activated over [~]5 min. Inhibiting or deleting Rab10 enhanced sLTP, electrophysiological LTP and AMPA receptor (AMPAR) trafficking during sLTP. In contrast, disrupting Rab4 impaired sLTP only in the first few minutes, and decreased AMPAR trafficking during sLTP. Thus, our results suggest that Rab10 and Rab4 oppositely regulate AMPAR trafficking during sLTP, and inactivation of Rab10 signaling facilitates the induction of LTP and associated spine structural plasticity.

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