Ryohei Yasuda

The paper develops a red fluorescent sensor, chILI, for detecting cAMP in living cells through intensity and lifetime imaging, demonstrating its use in mouse brain neurons.

The paper develops sensitive FRET biosensors to visualize CaMKII activity dynamics in living cells and demonstrates their use in cultured and primary cells, including neurons and cardiomyocytes.

The paper introduces Neuroplex, a method combining miniscope Ca2+ recordings with spectral imaging to identify nine neuronal subtypes in freely behaving animals.

This study shows that KIF11 mutations impair microtubule dynamics and dendritic arborization in neurons, contributing to intellectual disabilities.

This study shows that deleting ADAP1/CentA1 in Alzheimer's disease mice reduces cognitive impairment and neuropathological features, with transcriptome analysis identifying Bid as a key mediator.

The paper shows MMP-9 is essential for structural long-term potentiation by cleaving proBDNF into mature BDNF and activating TrkB.

The paper shows Rab10 inactivation enhances sLTP and AMPAR trafficking, while Rab4 activation has the opposite effect during synaptic plasticity.

The paper investigates how mitochondria near synapses remodel to increase ATP production and support synaptic plasticity through structural and functional changes.

This study investigates the sex-specific effects of ADEPTR loss of function on neuronal morphology and behavior in mice, revealing deficits in spine structure and anxiety in females.

This study shows that CaMKII inhibition impairs short-term but not long-term memory in an inhibitory avoidance task, suggesting long-term memory can form without short-term memory.

This study shows that cell-type-specific synaptic plasticity in PT neurons is essential for shaping neocortical dynamics during motor learning.

The paper investigates the molecular mechanisms of behavioral time scale plasticity (BTSP) in CA1 synapses, finding that delayed, dendritic, and stochastic CaMKII activation (DDSC) is essential for BTSP.

This study investigates the role of PKCδ in synaptic plasticity using FLIM-FRET sensors in mouse CA1 neurons, revealing its dual role in local and nuclear signaling.

This study develops a low-cost, efficient method to generate floxed mice using CRISPR-Cas9 and in vitro fertilization, achieving a 10% success rate in 125 days with 16 mice.

This study shows that IGF1 synthesized and released by CA1 pyramidal neurons triggers autocrine signaling that regulates dendritic spine plasticity in mice.

This study identifies Rac1 and Arp2/3 as key regulators of presynaptic plasticity through proteomic analysis and shows their coupling to action potentials via calcium influx.

The paper identifies an intronic lncRNA, ADEPTR, that is synaptically transported via cAMP/PKA and interacts with the Spectrin/Ankyrin network to regulate structural plasticity in hippocampal neurons.

This study shows PKCα activates Rac1 during structural synaptic plasticity, linking PKC to spine remodeling through a PDZ binding domain.

The paper uses high throughput correlative light-electron microscopy to analyze rapid ultrastructural changes in dendritic spines during LTP, finding rapid reorganization of the PSD and increased eASI area.

The paper introduces an open-source MATLAB tool using machine learning to automatically identify dendritic spines in fluorescent images with high accuracy.

This study shows RGS14 restricts synaptic plasticity in hippocampal CA2 by limiting postsynaptic calcium signaling and downstream pathways.

The paper introduces an automated system for tracking, imaging, and stimulating dendritic spines to improve throughput in structural plasticity studies.