iSODA: A Comprehensive Tool for Integrative Omics Data Analysis in Single- and Multi-Omics Experiments

Omics technologies including genomics, proteomics, metabolomics, and lipidomics allow profound insights into health and disease. Thanks to plummeting costs of continuously evolving omics analytical platforms, research centers collect multi-omics data more routinely. They are, however, confronted with the lack of a versatile software solution to harmoniously analyze single-omics data and merge and interpret multi-omics data. We have developed iSODA, an interactive web-based application for the analysis of single-as well as multi-omics omics data. The software tool emphasizes intuitive, interactive visualizations designed for user-driven data exploration. Researchers can filter and normalize their datasets and access a variety of functions ranging from simple data visualization like volcano plots and PCA, to advanced functional analyses like enrichment analysis for proteomics and saturation analysis for lipidomics. For insights from integrated multi-omics, iSODA incorporates Multi-Omics Factor Analysis - MOFA, and Similarity Network Fusion - SNF. All results are presented in interactive plots with the possibility of downloading plots and associated data. The ability to adapt the imported data on-the-fly allows for tasks such as removal of outlier samples or failed features, various imputation strategies, or data normalization. The modular design allows for extensions with new analyses and plots. The software is accessible under http://isoda.online/. O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=112 SRC="FIGDIR/small/605811v1_ufig1.gif" ALT="Figure 1"> View larger version (41K): org.highwire.dtl.DTLVardef@1eb61c8org.highwire.dtl.DTLVardef@60a7feorg.highwire.dtl.DTLVardef@1b54b41org.highwire.dtl.DTLVardef@15fd1b2_HPS_FORMAT_FIGEXP M_FIG C_FIG Graphical summary for iSODA showcasing some application examples, the data import, the single-omics and multi-omics modules.