SARS-CoV-2 detection with CRISPR diagnostics

Lu Guo; Xuehan Sun; Xinge Wang; Liang Chen; Haiping Jiang; Qingqin Gao; Moyu Dai; Bin Qu; Sen Fang; Yihuan Mao; Yangcan Chen; Guihai Feng; Qi Gu; Ruiqi Rachel Wang; Qi Zhou; Wei Li

doi:10.1038/s41421-020-0174-y

Molecular BiologyCell DiscoveryMay 18, 2020

Peer-reviewed journal article

SARS-CoV-2 detection with CRISPR diagnostics

Lu Guo iD, Xuehan Sun iD, Xinge Wang iD, Liang Chen iD, Haiping Jiang iD, Qingqin Gao, Moyu Dai, Bin Qu iD, Sen Fang, Yihuan Mao, Yangcan Chen, Guihai Feng iD, Qi Gu iD, Ruiqi Rachel Wang iD, Qi Zhou iD, Wei Li iD

Abstract

The novel coronavirus (CoV) disease termed COVID-19 (Coronavirus Disease-19) caused by SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus-2) is causing a massive pandemic worldwide, threatening public health systems across the globe. During this ongoing COVID-19 outbreak, nucleic acid detection has played an important role in early diagnosis. Here we report a SARS-CoV-2 detection protocol using a CRISPR-based CRISPR diagnostic platform - CDetection (Cas12b-mediated DNA detection). By combining sample treatment protocols and nucleic acid amplification methods with CDetection, we have established an integrated viral nucleic acid detection platform - CASdetec (CRISPR-assisted detection). The detection limit of CASdetec for SARS-CoV-2 pseudovirus is 1 x 104 copies/mL, with no cross reactivity observed. Our assay design and optimization process can provide guidance for future CRISPR-based nucleic acid detection assay development and optimization.

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