Screening of anti-mycobacterial compounds in a naturally infected zebrafish embryo model

Abstract

Synopsis Synopsis Introduction Materials and Methods Results Discussion Funding Transparency statement References Mycobacterium tuberculosis is a deadly human pathogen that latently infects a third of the world's population, resulting in approximately 1.5 million deaths per year. Due to the difficulties and expense of carrying out animal drug trials using M. tuberculosis and rodents, infections of the zebrafish Danio rerio with M. marinum have been used as a surrogate. However the methods so far described require specialised equipment and a high level of operator expertise.\n\nWe investigated a natural infection model where zebrafish embryos are infected through incubation in media containing M. marinum. Using bioluminescently labelled ...