An improved MS2-MCP imaging system with minimal perturbation of mRNA stability
Abstract
The MS2-MCP imaging system is widely used to study the mRNA spatial distribution in living cells. Here, we report that the MS2-MCP system may destabilize the tagged mRNA by targeting it to the nonsense-mediated mRNA decay pathway. We introduce an improved version, which has minimal perturbation of the mRNA stability.
02 /Specification & Classification
Click any tag to browse matching papers- Assay
- Approach
Reproducibility ledger
Nobody has reported trying to reproduce this paper yet. Did you replicate an experiment or run the code? Your report saves the next lab a week.
Sign in to file a reproducibility report.
Related papers
- RNA-Stabilized Coat Proteins for Sensitive and Simultaneous Imaging of Distinct Single mRNAs in Live Cells
Christopher J. Kuffner, Alexander M. Marzilli, John T. Ngo·Nature MethodsPeer-reviewed·Sep 22, 2025
- An adapted MS2-MCP system to visualize endogenous cytoplasmic mRNA with live imaging in Caenorhabditis elegans
Cristina Tocchini, Susan E. Mango·bioRxiv·Jun 13, 2023
- Orthogonal RNA-regulated destabilization domains for three-color RNA imaging with minimal RNA perturbation
Tien G. Pham, Omoyemi Ajayi, … Jiahui Wu·Nature MethodsPeer-reviewed·Dec 17, 2025
- Establishing MS2-MCP-based single-molecule RNA visualization in Schizosaccharomyces pombe
Douglas E. Weidemann, Sarah C Turner, … Silke Hauf·Journal of Cell SciencePeer-reviewed·Jul 1, 2026
- SunRISE: long-term imaging of individual mRNA molecules in living cells
Y. Guo, R. E. C. Lee·Cell Reports MethodsPeer-reviewed·Jun 1, 2022
- Nuclear-BRITE: an enhanced MS2 system for live-cell imaging of nuclear RNAs
Adam D. Cawte, Bara Cihlova, … Neil Brockdorff·bioRxiv·Oct 9, 2026