R. H. Singer

Transcriptome-wide mRNP condensation precedes stress granuleformation and excludes new mRNAs

The paper shows that mRNAs condense broadly in yeast under stress, independent of length, and new transcripts escape this condensation for selective translation.

A series of spontaneously blinking dyes for super-resolution microscopy

This paper introduces a series of spontaneously blinking dyes with adjustable duty cycles for improved single-molecule localization microscopy in cells and dense structures.

Lysosomal release of amino acids at ER three-way junctions regulates transmembrane and secretory protein mRNA translation

The paper shows that lysosomal amino acid release at ER junctions regulates translation of secretome mRNAs, with Lunapark and lysosomal function playing key roles.

An improved MS2-MCP imaging system with minimal perturbation of mRNA stability

The paper identifies that the MS2-MCP system can destabilize mRNA and introduces an improved version with minimal mRNA stability perturbation.

Global analysis of human-to-mouse contact-dependent intercellular mRNA and lncRNA transfer in cell culture

The paper quantifies mRNA and lncRNA transfer between human and mouse cells in co-culture, showing non-selective transfer and correlation with donor gene expression.

Bromodomains regulate dynamic targeting of the PBAF chromatin remodeling complex to chromatin hubs

This study uses live-cell imaging to show that PBAF chromatin remodeler dynamically targets acetylated chromatin hubs via bromodomains, with distinct binding kinetics in euchromatin and heterochromatin.

Transcriptional burst kinetics are linked to short term transcriptional memory

The paper studies transcriptional bursting at the p21 gene and identifies short-term transcriptional memory (STTM) linked to p53 regulation and burst duration.

Cap-dependent translation initiation monitored in living cells

The paper uses live-cell imaging to study the dynamics of translation initiation factors at the 5' cap, revealing their temporal regulation.

Cellular variability of nonsense-mediated mRNA decay

The paper uses a single-cell reporter system to show cell-to-cell variability in NMD efficiency, linked to differences in SMG1 and phosphorylated UPF1 levels.

Mitochondrial volume fraction and translation speed impact mRNA localization and production of nuclear-encoded mitochondrial proteins

The structural basis for RNA selectivity by the IMP family of RNA binding proteins

This study identifies the RNA binding specificity of IMP family proteins using SELEX and NMR, showing that variable loops determine target RNA selection and disease-related functions.

Re-targeting of macroH2A following mitosis to cytogenetic-scale heterochromatic domains

This study shows that macroH2A re-targets to heterochromatic domains in G1 phase after DNA replication, maintaining epigenetic memory through cell division.

Intercellular mRNA Trafficking Via Membrane Nanotubes In Mammalian Cells

This paper demonstrates that full-length mRNAs transfer between mammalian cells via membrane nanotubes, not extracellular vesicles, and shows this process alters the acceptor cell proteome.

Nuclear Microenvironments Modulate Transcription From Low-Affinity Enhancers

The paper shows that low-affinity enhancer binding by Ubx is efficient in nuclear microenvironments with high Ubx concentration, aided by cofactor Hth.